Ificant in the hypothermia group (Table 1).Table 1 (abstract P462) Characteristic Number of patients Median age (years [range]) Type of cardiac arrest Asystole PEA VF Sex Witnessed collapse Bystander - CPR Median interval collapse to ROSC (min [range]) Median tympanic temperature at admission ( ) Outcome CPC 1? CPC 3? CPC 5 Death of other origin than cerebral Hypothermia group 14 58 (11?1) 7 2 5 7M/7F 10 9 24 (10?5) 36 (32.5?7.7) Normothermia group 14 74 (59?1) 12 2 0 9M/5F 6 2 33 (25?0) 35.5 (33.7?6.7)Methods Post-hoc analysis of data of the HACA multicenter trial for patients included at our study center. Patients after ventricular fibrillation cardiac arrest were randomly assigned to mild therapeutic hypothermia of 32?4 over 24 hours or to conventional treatment. For the present analysis we analysed the effect on plasma levels of CK and CKMB as a measure of infarct size. Results Fifty-five patients underwent cooling and 56 patients received standard treatment after successful resuscitation. The analysis was performed according to the intention-to-treat principle. The areas under the curve (AUC) within 24 hours for CK were 28,786 U/l ?24 hours (IQR 5646?4,998) in the cooling group and 20,373 U/l ?24 hours (IQR 8211?0,801) for controls (P = 0.40). For CK-MB the AUC was 1690 U/l ?24 hours (IQR 724?330) in the cooling group and 1187 U/l ?24 hours (IQR 490?469) for controls (P = 0.18). Conclusion In our sample cooling after successful resuscitation for ventricular fibrillation cardiac arrest did not influence infarct size as estimated by CK and CK-MB levels.P464 Neuron-specific enolase and protein S-100 B as prognostic parameters for children with head injuryM Scheler-Hofmann, T Howell, E Panzig, C Fusch, R Stenger University of Greifswald, Germany Critical Care 2006, 10(Suppl 1):P464 (doi: 10.1186/cc4811) Introduction Neuron-specific enolase (NSE) (glycolytic enzyme in neurons and neuroendocrine cells) and protein S-100 B (calcium2+binding protein in astroglial and Schwann cells) are established PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27509597 neurobiochemical markers that can be used for evaluating the severity of organic brain damage. The examination of these parameters in serum was used to show the practical relevance in correlation with further examination criteria (Glasgow Coma Scale [GCS], outcome, hospital treatment and ventilation time). Patients and methods Thirty-nine boys and 16 girls aged 8 ?4.7 years (range 2 months?6 years) suffering from light to severe head injury (49.1 traffic accident, 38.2 falls, 3.6 child abuse, 3.6 riding accidents, 5.5 other accidents) were included in the study. The examinations of NSE (reference 12 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/7891282 points), the hospital treatment time, the ventilation hours and the outcome were tested towards significance (Mann hitney test). Results Significant correlations for NSE and protein S-100 B were (S,R,S)-AHPC-C7-amine seen for the outcome of patients with head injury. NSE (P < 0.01) and protein S-100 B (P < 0.05) were significantly higher when rehabilitation was.